Zinc affects nuclear factor kappa b and DNA methyltransferase activity in C3H cancer fibroblast cells induced by a 2100 MHz electromagnetic field.

Duzgun Ergun D., Pastaci Ozsobaci N., Yilmaz T., Ozcelik D., Kalkan M. T.

Electromagnetic biology and medicine, cilt.41, sa.1, ss.93-100, 2022 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1080/15368378.2021.2019760
  • Dergi Adı: Electromagnetic biology and medicine
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Compendex, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.93-100
  • Anahtar Kelimeler: Cancer, DNMT, mobile phone, NF-kappa B, Zinc, RADIOFREQUENCY RADIATION, OXIDATIVE STRESS, PROLIFERATION, PHONE
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet

Özet

Zinc affects nuclear factor kappa b and DNA methyltransferase activity in C3H cancer fibroblast cells induced by a 2100 MHz electromagnetic field

D. Duzgun Ergun a, N. Pastaci Ozsobaci b, T. Yilmazc, D. Ozcelik b, and M. T. Kalkan a

aDepartment of Biophysics, Faculty of Medicine, Istanbul Aydin University, Istanbul, Turkey; bDepartment of Biophysics, Cerrahpasa Medical

Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey; cDepartment of Electronics and Communication Engineering, Istanbul Technical

University, Istanbul, Turkey

ABSTRACT

The use of mobile phones is becoming widespread with the development of technology, and as

a result, its effects on human health are becoming more and more important every day. Studies

have reported that the electromagnetic field (EMF) emitted by mobile phones may have adverse

effects on the biological systems. In order to evaluate the effect of zinc (Zn) on C3H cancer fibroblast

cells exposed to 2100 MHz EMF, we analyzed cell viability%, nuclear factor kappa b (NF-κB) and DNA

methyltransferase (DNMT) activities. Cells were divided to following groups: Control, sham control,

2100 MHz EMF, 50 μM Zn + 2100 MHz EMF, 100 μM Zn + 2100 MHz EMF, and 200 μM Zn + 2100 MHz

EMF for 2 h. We measurement cell viability, NF-κB and DNMT activities. There was increased cell

viability % in the 2100 MHz EMF group compared to the control group, while the cell viability % was

decreased in the 50, 100 and 200 μM Zn + 2100 MHz EMF groups compared to 2100 MHz EMF. NF-

κB and DNMT activities were a significant increase in the 2100 MHz EMF group compared to the

control group, although were statistically decreased in the 50, 100 and 200 μM Zn + 2100 MHz EMF

groups compared to the 2100 MHz EMF group. Our results demonstrate that 2100 MHz EMF

exposure in cancer fibroblast cells induce NF-κB and DNMT activities, whereas zinc supplementation

reduce NF-κB and DNMT activities-induced 2100 MHz EMF.