Early pregnancy diagnosis by semiquantitative evaluation of luteal vascularity using power Doppler ultrasonography in sheep


Creative Commons License

Dal G. E., Enginler S. Ö., Baykal K., Sabuncu A.

ACTA VETERINARIA BRNO, vol.88, pp.19-23, 2019 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 88
  • Publication Date: 2019
  • Doi Number: 10.2754/avb201988010019
  • Journal Name: ACTA VETERINARIA BRNO
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.19-23
  • Keywords: Area of coloured pixels, ultrasound examination, corpus luteum, ovine, BLOOD-FLOW, SUBJECTIVE ASSESSMENT, EWES
  • Open Archive Collection: AVESIS Open Access Collection
  • Istanbul University-Cerrahpasa Affiliated: Yes

Abstract

The aim of the study was to measure the area of coloured pixels captured in power Doppler examination of corpus luteum (CL) by a computer-assisted image analysis software and to evaluate the efficiency of the semiquantitative assessment of CL in early pregnancy diagnosis in sheep. Assessment of luteal vascularity was carried out on 30 Kivircik ewes with power Doppler ultrasonography daily from Day 12 to Day 19 post breeding. The mean area of coloured pixels of CL with pregnancy was found significantly higher than the area of coloured pixels of CL undergoing luteolysis (0.38 +/- 0.012 mm(2) and 0.31 +/- 0.017 mm(2), respectively, P < 0.001). Assessment of day-based examination showed a significant difference between the area of coloured pixels of CL with continuing pregnancy and luteolysis on Day 17 (P < 0.05), which was followed by Days 18 and 19 (P < 0.001). It is concluded that pregnancy diagnosis can be done accurately by semiquantitative assessment of luteal vascularization as early as 17 days post breeding in ewes. According to the authors' knowledge, this is the first report on the efficacy of semiquantitative assessment of ovine CL in early pregnancy diagnosis.