Presence of Extended-Spectrum Beta-Lactamase-producing Escherichia coli and carbapenem resistance in ready-to-eat stuffed mussels in Istanbul

Aydın A.

ACTA ALIMENTARIA, cilt.53, sa.2, ss.200-213, 2024 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 53 Sayı: 2
  • Basım Tarihi: 2024
  • Dergi Adı: ACTA ALIMENTARIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.200-213
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır

Özet

Foodborne pathogens transmission is essential in the spread of antibiotic resistance, and extended-spectrum beta-lactamase-producing Escherichia coli especially threatens public health. E. coli is essential to resistance to commonly used beta-lactam group antibiotics. Ready-to-eat stuffed mussels are a popular food from many restaurants and street vendors, including potential health risks for food hygiene. 200 RTE stuffed mussels were collected from the Asian and European sides of Istanbul and analyzed for the presence of E. coli. As a result of PCR analysis,  E. coli was detected in 7 (3.5%) samples. An antibiotic susceptibility test was performed using the disc diffusion method to determine ESBL and carbapenem resistance. All isolates were resistant to ampicillin. The double-disk synergy test was performed as an ESBL phenotypic confirmation test, and no phenotypically ESBL-producing E. coli were detected. The blaTEM gene (14.2%) was detected in one isolate by mPCR, but blaCTX-M, blaSHV, and blaOXA genes were not observed. Meropenem and imipenem were used for the disk diffusion method for carbapenem resistance, and no resistant isolate was found. Carbapenem resistance genes were investigated by monoplex PCR, and blaNDM-1, blaOXA-48, blaVIM, and blaIMP resistance genes were not detected. The detection of ESBL-producing E. coli in RTE stuffed mussels for the first data in Türkiye could represent a public health risk.