Akademik Veri Yönetim Sistemi
The Dangers of Psychoactive Substances, Denise J. Burton, Editör, NOVA Science Publishers Inc. , New York, ss.23-42, 2023
Caffeine is one of the
most widely used pharmaceutics and preferred as an anthropogenic marker to
indicate only human activities in water samples. Therefore, developing a fast
and reliable analytical method for caffeine in water is necessary and crucial.
In this study, it was aimed to develop and comprehensively validate a method conducted
with Liquid Chromatography- Tandem Mass Spectrometry system. Validation
parameters such as selectivity, analytical sensitivity, limit of detection and
quantification, linearity, precision, accuracy and robustness were studied
beside uncertainty. Correlation coefficient of calibration curve was found 0.997
whilelimit of detection and limit of quantification levels were measured as
0.165 and 0.548 ng/mL respectively. Repeatability studies were found below 10%
RSD. Accuracy was between 87-109% in different days and at different
concentrations in the range of 0.5-100 ng/mL.After the method validation, tap
water samples (n=6) were enriched with concentrations of 50 ng/mL in 50 mL and
solid phase extraction (SPE) recovery was found as 93.3%. This is the first
comprehensively developed and validated method published in our country to
detect caffeine in water with high precision, accuracy and recovery. The method
can be easily applied to sediment, ground or seawater samples besides surface
water and wastewater.