Kidney Damage Related with Pulmonary Toxicity in Mice

Ertik O., Yıldırım M., Yanardağ R., Öztay F.

5th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2022), Ankara, Türkiye, 23 - 25 Kasım 2022, ss.58, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.58
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet

Özet

Bleomycin (BLM), an antitumor effective drug used in cancer treatment, often causes pulmonary toxicity due to use, and

therefore, studies are continuing to reduce this effect in research subjects due to its strong side effects. In addition, patients

treated with BLM may develop pulmonary fibrosis. More recently, an increased prevalence of chronic kidney disease has

been reported in elderly people with pulmonary fibrosis (IPF). Administration of atorvastatin (ATR) to mice and rats

with pulmonary fibrosis or lung fibroblasts reduces fibrosis formation. The current study was designed to investigate the

protective effects of ATR on renal oxidative damage in kidney tissue of mice with pulmonary fibrosis. Adult male

C57BL/6J mice were used in the present study and divided into four groups: (1) control group, (2) ATR group, (3) BLM

group, and (4) BLM + ATR group. At the end of the experiment, kidney tissues were taken and homogenized in cold

0.9% NaCl with glass equipment to obtain 10% (w/v) homogenate and centrifuged at 10.000g at +4°C for 10 min. The

supernatants were used for biochemical analysis. Reduced glutathione (GSH), lipid peroxidation (LPO), total antioxidant

status (TAS), total oxidant status (TOS), reactive oxygen species (ROS), oxidative stress index (OSI) levels and lactate

dehydrogenase (LDH), glutamate dehydrogenase (GDH) and arginase activities in addition to the presence of cell death

were determined in kidneys tissues. In BLM groups, LPO, TOS, ROS, OSI levels, and LDH activity were found to be

increased, while GSH, TAS, Bax and active-caspase-3 levels, and GDH and arginase activities were decreased. The

administration of ATR to mice treated with BLM reversed these changes but, apoptosis was induced in kidney of these

mice. These results show to be the ameliorative effect of ATR only on oxidative damage in kidney tissue of mice with

pulmonary fibrosis.