Development of a densitometric HPTLC method for determination of 5-HMF in fruit-based baby foods in Turkey

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Turkmen Z.

JOURNAL OF CHEMICAL METROLOGY, cilt.14, sa.1, ss.52-62, 2020 (ESCI, Scopus, TRDizin) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 1
  • Basım Tarihi: 2020
  • Doi Numarası: 10.25135/jcm.39.20.05.1653
  • Dergi Adı: JOURNAL OF CHEMICAL METROLOGY
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.52-62
  • Anahtar Kelimeler: Baby food, 5-hydroxymethylfurfural, HPTLC, method validation, QuEChERS, LIQUID-CHROMATOGRAPHIC DETERMINATION, FURFURAL COMPOUNDS, INFANT FORMULAS, ACID, FURAN, HONEY, JAMS
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet

Özet

This study reports the determination of 5-hydroxymethylfurfural (5-HMF) by HPTLC in fruit-based baby food products and compares the other techniques. The performance of QuEChERS method was evaluated for the measurement of 5-HMF. The mean recovery and RSD% at the two-fortification level of 25 and 250 ng/spot were 83.65% and 2.0%, and 96.02% and 3.0%, respectively. LOD and LOQ levels for 5-HMF were found 1.54 ng/spot and 4.67 ng/spot in fruit-based baby foods, respectively. Fifty eight commercial products on the Istanbul market were evaluated by developed and validated HPTLC method and, 5 -HMF were determined in 48 (82.76%) in the range of 13.12-28.90 mg/kg. Although the concentration of 82.76% of the 5-HMF detected products was found to be below the legal limit, the results revealed that a new regulation should be made in fruit-based food considering the baby exposure limit. The analytical performance of this method verifies its potential applicability with particularly low cost and simple sample pretreatment.