Prevalence and Phylogenetic Groups of E. coli in Raw Frozen Chicken Meat from Istanbul

Süleymanoğlu A. A., Aydın A.

20th International Scientific Conference "Tradition and Modernity in Veterinary Medicine", Sofija, Bulgaristan, 25 - 27 Nisan 2025, ss.46, (Tam Metin Bildiri)

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: Sofija
  • Basıldığı Ülke: Bulgaristan
  • Sayfa Sayıları: ss.46
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet

Özet

Escherichia coli is a bacterium that poses a threat to food safety and public health due to its diverse pathogenic subtypes. This study investigated the prevalence of E. coli in 597 samples of frozen raw chicken meat collected from retail outlets in Istanbul, Türkiye, in 2013. Both conventional microbiological methods and PCR were used for the analysis. E. coli-specific 16S rRNA and the GyrB gene were confirmed in 6.7% (40 out of 597) of the E. coli isolates. E. coli isolates from 8 phylogroups (A, B1, B2, C, D, E, F, and Escherichia cryptic clade I) were investigated using PCR. For phylogenetic analysis of E. coli isolates, the presence of the chuA, yjaA, TspE4.C2, arpA, and trpA genes, as well as their interrelationships, was assessed. According to the PCR results, phylogenetic groups A, B2, C, D, and E accounted for 15%, 2.5%, 35%, 12.5%, and 17.5%, respectively, with 17.5% of the isolates remaining untyped. No E. coli isolates belonging to phylogenetic groups B1, F, and cryptic clade 1 were detected. According to the results, E. coli isolates may potentially be commensal strains. This study comprehensively analyzed the prevalence and phylogenetic grouping of E. coli in chicken meat from Istanbul, a major metropolitan city at the crossroads of Asia and Europe.