Hypericum perforatum Oil and Vitamin A Palmitate-Loaded Gelatin Nanofibers Cross-Linked by Tannic Acid as Wound Dressings
ACS OMEGA, cilt.8, sa.26, ss.24023-24031, 2023 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 8 Sayı: 26
- Basım Tarihi: 2023
- Doi Numarası: 10.1021/acsomega.3c02967
- Dergi Adı: ACS OMEGA
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Directory of Open Access Journals
- Sayfa Sayıları: ss.24023-24031
- İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır
Özet
Recent studies in wound dressing applications offer newtherapiesto promote the wound healing process. The main strategy of this studyis to combine the traditional perspective of using medicinal oilswith polymeric scaffolds manufactured by an engineering approach tofabricate a potential tissue engineering product that provides bothnew tissue formation and wound healing. Thus, Hypericumperforatum oil (HPO) and vitamin A palmitate (VAP)incorporated gelatin (Gt) nanofibrous scaffolds were successfullyprepared by the electrospinning method. Tannic acid (TA) was usedas the cross-linking agent. The amounts of VAP and HPO loaded in thebase Gt solution [15% w/v in 4:6 v/v acetic acid/deionized water]were 5 and 50 wt % (based on the weight of Gt), respectively. Theobtained scaffolds were studied regarding their microstructure, chemicalstructure, thermal stability, antibacterial activity, in vitro releasestudy, and cellular proliferation assay. In the light of these studies,it was determined that VAP and HPO were incorporated successfullyin Gt nanofibers cross-linked with TA. Release kinetic tests confirmedthat the patterns of TA and VAP release were consistent with the Higuchimodel, whereas HPO release was consistent with the first-order kineticmodel. In addition, this membrane was biocompatible with L929 fibroblastcells and had antibacterial activity and thermal stability. This preliminarystudy suggests potential applicability of the proposed dressing totreat skin wounds in clinics.