Akademik Veri Yönetim Sistemi
XXXI MEETING OF BALKAN CLINICAL LABORATORY FEDERATION 35th NATIONAL BIOCHEMISTRY CONGRESS, Antalya, Türkiye, 28 Ekim - 01 Kasım 2024, cilt.49, sa.1, ss.197-198, (Özet Bildiri)
Objectives: In this study, we investigated the effects of Alpha Melanocyte Stimulating Hormone(α-MSH) on neurogenesis and NGF, BDNF, IL-10 expressions in olfactory epithelium(OE) in peripheral olfactory disorder and investigated the possible role of α-MSH in olfactory mechanisms and treatment.
Methods: To model an odor disorder, 48 rats were divided into two groups: one received intranasal 10% zinc sulfate, the other intranasal saline. Each group was further divided, with half receiving α-MSH (5 mg/ kg) and the others saline intraperitoneally, followed by euthanasia on days 4 and 21. Olfactory function was assessed via buried food and odor discrimination tests at baseline (T0), after treatment (T1), and before euthanasia (T2). Blood samples were analyzed by ELISA, and nasal tissues were examined by immunohistochemistry.
Results: The 21-day α-MSH treatment(OD+α-MSH group) in rats with olfactory disorder (OD) resulted in an increase in ASCL1+,GAP-43+,OMP+ immunoreactivities and OE thickness and had positive effects on OE neurogenesis. On day 21, IL-10 immunoreactivity in the OD+α-MSH group showed a non-significant increase compared to the OD group and a significant increase compared to the control group(p < 0.05). There was no increase in NGF+ and BDNF+ immunoreactivity in the OD+α-MSH group compared to the OD group. In the OD+α-MSH group, better results were obtained in odor tests, especially on day 21.
Conclusions: 21-day α-MSH treatment may positively affect OE neurogenesis by increasing stem cells, immature neurons and mature neurons in OE. The effect of α-MSH on OE neurogenesis may be direct and/or indirect through its anti-inflammatory effects.