DNA Probing of Peroxidase-Mimetic Activities of Selected Manganese-Based Nanozymes and Measurement of Antioxidant Activity for DNA Protection
ChemistrySelect, cilt.9, sa.38, 2024 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 9 Sayı: 38
- Basım Tarihi: 2024
- Doi Numarası: 10.1002/slct.202403369
- Dergi Adı: ChemistrySelect
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier
- Anahtar Kelimeler: CUPRAC method, DNA protection, Manganese-based nanozymes, Peroxidase, TMB method
- İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet
Özet
Three different Mn-based peroxidase-mimetic nanoparticles (NPs) were synthesized as nanozymes: (i) manganese (II)-ferrite (MnFe2O4) fabricated by co-precipitating MnCl2.4H2O and FeCl3.6H2O; (ii) MnO2 synthesized from precipitation of MnSO4 in basic medium; (iii) manganese oxide obtained by reducing KMnO4 with tris(2-hydroxyethyl) amine, all characterized using STEM, EDXS, FTIR and XPS techniques. These NPs acted as peroxidases, revealed by using a DNA probe, on which the damage generated by peroxidase mimetic NPs was monitored spectrophotometrically by CUPRAC and 3,3′,5,5′-tetramethylbenzidine (TMB) methods. Results were confirmed by RP-HPLC and voltammetry, the latter involving an AuNPs-modified glassy carbon electrode (GCE), multi-walled carbon nanotubes (MWCNT) and Nafion combination. Colorimetric assay with CUPRAC rather than TMB better agreed with chromatographic/voltammetric findings in detecting both peroxidase activity and DNA hazard. Mn(III)-containing manganese oxide from permanganate reduction had stronger peroxidase activity. The proposed method demonstrated protective effects of antioxidants (morin, catechin and quercetin) against oxidative DNA damage for the first time.