Magneto-electrochemical biosensing for pathogen detection using nuclease-responsive nanohybrids
Microchimica Acta, cilt.193, sa.5, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 193 Sayı: 5
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s00604-026-08087-3
- Dergi Adı: Microchimica Acta
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, Chimica, Compendex
- Anahtar Kelimeler: Electrochemical biosensor, Emulsion polymerization, Green synthesis, Nuclease-based detection, POSS, Streptococcus pneumoniae, Superparamagnetic iron oxide nanoparticles, UV-polymerization
- İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet
Özet
The development of sustainable and highly sensitive diagnostic platforms is critical for rapid pathogen identification and effective disease management. Here, a green, magneto-electrochemical biosensing strategy is reported for the selective detection of Streptococcus pneumoniae based on pathogen-specific nuclease activity. Uniform organic–inorganic hybrid polyhedral oligomeric silsesquioxane (POSS) nanoparticles were synthesized via an ultrafast UV-initiated emulsion polymerization within 5 min using an eco-friendly approach. The nanoparticles were sequentially functionalized by in situ deposition of superparamagnetic iron oxide nanoparticles and biomimetic polydopamine coating, enabling robust and high-density immobilization of nuclease-responsive oligonucleotide probes. The resulting PDA@SPION/POSS nanohybrids exhibit controlled size, preserved structural integrity, and strong superparamagnetic behavior, allowing efficient magnetic manipulation and electrochemical signal transduction. Upon exposure to S. pneumoniae, nuclease-mediated probe cleavage produces a pronounced electrochemical response, enabling label-free detection over a wide dynamic range (102–10⁸ CFU mL⁻¹) with a detection limit of 102 CFU mL⁻¹. High selectivity against non-target bacteria highlights the specificity of the enzymatic recognition mechanism. This work establishes a sustainable and amplification-free biosensing platform with strong potential for rapid clinical diagnostics.