Development of a Rapid Molecular Test Format Based on Isothermal Recombinase Polymerase Amplification Technique and Lateral Flow Method for Detection of bla(OXA-48)

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Kuskucu M. A., Aygun G., Karakullukcu A., Imamova N., Kucukbasmaci Ö., Midilli K.

KLIMIK JOURNAL, cilt.31, sa.3, ss.185-189, 2018 (ESCI, Scopus, TRDizin) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 3
  • Basım Tarihi: 2018
  • Doi Numarası: 10.5152/kd.2018.46
  • Dergi Adı: KLIMIK JOURNAL
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.185-189
  • Anahtar Kelimeler: bla(OXA-48), isothermal amplification recombinase polymerase amplification technique, lateral flow method, RESISTANT KLEBSIELLA-PNEUMONIAE, CARBAPENEM RESISTANCE, SPREAD, ASSAY, ENTEROBACTERIACEAE, ISTANBUL, OXA-48
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır

Özet

Objective: Molecular tests are rapid, reliable tools for the detection of carbapenem resistance, but their use is limited due to their cost, requirement for well-trained technicians and highly sophisticated instrumentation. The recombinase polymerase amplification (RPA) assay, one of the isothermal amplification methods developed recently to overcome these problems, doesn't require denaturation of target, and RPA products can be determined by probe-base detection methods even without a specific instrumentation. In this study, we aimed to develop a rapid and easily applicable molecular test format in on-site settings based on RPA technique with combination of lateral flow system for detection of bla(OXA-48).