Akademik Veri Yönetim Sistemi
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, cilt.84, 2023 (SCI-Expanded, Scopus)
This study aims to produce microemulsion formulations containing naringenin for anti-aging reasons. For this purpose, formulations of topical microemulsions including different cosurfactants and surfactants were devel-oped, and a triangle phase diagram was utilized to determine the optimal microemulsion system. On the developed microemulsion formulations, characterization studies were performed. Ex vivo permeation -penetrating and in vitro release experiments were conducted. Anti-elastase, anti-collagenase, anti-tyrosinase, and antioxidant properties of the formulations were also evaluated. Experiments were conducted on cytotox-icity, cellular ROS, gene expression levels of oxidative stress-related genes, and cell culture. Droplet size, poly-dispersity index, zeta potential, pH, viscosity, turbidity of naringenin loaded formulations were found between 0.9147 +/- 0.0316 and 1.938 +/- 0.085 nm, 0.373 +/- 0.025 and 0.410 +/- 0.019,-0.0517 +/- 0.0138 and-0.0990 +/- 0.0110 mV, 5.110 +/- 0.006 and 5.530 +/- 0.006, 163.33 +/- 5.77 and 450.00 +/- 0.00 cP, 5.723 +/- 0.068 and 9.323 +/- 0.264 NTU, respectively. At the end of 24 h, the in vitro release research shows that more than 50% of naringenin has been released. Elastase and collagenase enzyme inhibitions of naringenin loaded formulations were found between 31.98 +/- 0.15 and 41.74 +/- 0.09%, 39.99 +/- 0.19 and 47.78 +/- 0.04%, respectively. Furthermore, NRF-2 and HO-1 gene expression levels did not show a significant change in the cells exposed to only naringenin loaded formulations. To conclude, developed microemulsions seem to be promising formulations that can be used for anti-aging purposes.