Akademik Veri Yönetim Sistemi
VETERINARY SCIENCES, cilt.12, sa.10, ss.1-14, 2025 (SCI-Expanded, Scopus)
Freezing and thawing semen are commonly used in animal breeding but often reduce sperm quality, which can affect fertility. Scientists are looking for simple ways to predict semen quality after thawing so that only the best samples are used for reproduction. In this study, we examined a protein found in sperm called precursor A-kinase anchor protein 4. We wanted to see whether the level of this protein could tell us something about how healthy the sperm would be after thawing. Semen was collected from goats, frozen, and then thawed. We measured the amount of this protein and compared it with many features of sperm, such as their ability to move, stay alive, and keep their cell structures intact. We found that sperm samples with higher amounts of the protein showed better movement, higher survival, stronger cell membranes, and more active energy production. However, one structure of the sperm, called the acrosome, was not affected. These results suggest that measuring this protein can help identify high-quality semen samples after freezing and thawing, which may improve breeding success and benefit animal production.