Akademik Veri Yönetim Sistemi
Euroanalysis2019 (XX. European Conference on Analytical Chemistry), İstanbul, Türkiye, 1 - 05 Eylül 2019, ss.160, (Özet Bildiri)
Determination of common inorganic anions and organic acids in food and beverages is an important issue in terms of nutritional, toxicological and monitoring of the product quality. Organic acids define the organoleptic quality and may also influence the stability of the product. A novel analytical method for simultaneous determination of fluoride, acetate, formate, chloride, nitrate and sulphate in distilled alcoholic beverages using ion chromatography with suppressed conductivity dedection was developed. Bromide internal standard was used as a strategy to improve the stability and accuracy of the method. The separation of fluoride, acetate, formate, chloride, nitrate and sulphate from distilled alcoholic beverages was accomplished by using an anion exchange column with optimized multistep gradient eluent program which utilized 5mM to 50 mM NaOH. The other chromatographic conditions were as follows: the suppressor current was 31 mA; the flow rate of mobile phase was 0.25 mL min⁻¹; the column and detector compartment temperature were 35 °C and 40°C respectively; sample loop volume was 10 μL. Six anion were separated completely within 20 minutes. All the curves showed good linearity (r² ≥ 0.999) within the calibration ranges. Under optimized conditions, the limits of detection (LODs) were between 0.56 and 13 μg L⁻¹. To evaluate the recovery test, three different concentrations of standard solutions were added to sample and the average recoveries were between 95.31 and 101.73%. Precision (RSD) was evaluated by continuously performing six replicates each day within 3 days for the determination of the three spiked samples. The relative standard deviations (RSDs) were less than 4.23 %. The results indicated that this method was simple, rapid, sensitive, and accurate for the determination of fluoride, acetate, formate, chloride, nitrate and sulphate in various distilled alcoholic beverages. Under the optimized conditions, actual concentrations of the selected anions were determined by the developed IC method in three kinds of commercial samples: Turkish raki, vodka and gin. The experimental results showed that the proposed method has a number of advantages such as easy to operate, good repeatability and low LODs. The mobile phase without adding any organic modifier made it environmentally friendly. In conclusion, the proposed method can be applied to monitor the selected ions in various distilled alcoholic beverages for routine analyses.