Horse Meat Microbiota: Determination of Biofilm Formation and Antibiotic Resistance of Isolated Staphylococcus spp.

Aydin A.

FOODBORNE PATHOGENS AND DISEASE, cilt.21, sa.10, ss.643-657, 2024 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 21 Sayı: 10
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1089/fpd.2023.0171
  • Dergi Adı: FOODBORNE PATHOGENS AND DISEASE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.643-657
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır

Özet

Domestic horses could be bred for leisure activities and meat production, as is already the case in many countries. Horse meat is consumed in various countries, including Kazakhstan and Kyrgyzstan, and with the increase in this consumption, horses are registered as livestock by the Food and Agricultural Organization. In this study, horse meat microbiota of horse samples (n=56; 32 samples from Kazakhstan and 24 samples from Kyrgyzstan) from 4 different regions, Kazakhstan (n=3) and Kyrgyzstan (n=1) were investigated first time by NGS and metabarcoding analysis. The results demonstrated that Firmicutes Proteobacteria and Actinobacteria were the dominant bacterial phylum in all samples. In addition, three (5,4%) Staphylococcus spp. were isolated from the Uzynagash region, Kazakhstan. Staphylococcus strains were identified as Staphylococcus warneri, S. epidermidis, and S. pasteuri by partial 16S rRNA DNA gene Sanger sequencing. All three Staphylococcus isolates were non-biofilm formers; only the S. pasteuri was detected as multidrug-resistant (resistant to penicillin, cefoxitin, and oxacillin). In addition, S. pasteuri was found to carry mecA, mecC, and tetK genes. Our first data indicated the presence of potential pathogen Staphylococcus spp. involved in horse meat samples originating from Kazakhstan. In conclusion, it should be carefully considered that undercooked horse meat may pose a risk to consumers in terms of pathogens such as antibiotic-resistant Staphylococcus isolates.