Akademik Veri Yönetim Sistemi
POLISH JOURNAL OF VETERINARY SCIENCES, cilt.27, sa.1, ss.13-23, 2024 (SCI-Expanded, Scopus)
Listeria monocytogenes is a ubiquitous microorganism that is isolated from a variety
of sources such as soil, water, decaying vegetation, sewage, animal feeds, silage, farm environments and food-processing environments. This study aimed to determine the prevalence, serogroups, biofilm formation, virulence factor genes, and genetic relationships of L. monocytogenes
strains isolated from beef meat and meat contact surfaces obtained from a slaughterhouse
in Burdur, Turkey. In this study, a total of 179 beef meat and meat contact surface samples
were analyzed for the presence of L. monocytogenes by polymerase chain reaction (PCR).
Out of a total of 179 beef meat and meat contact surface samples, 83 (46.37%) were found
to be contaminated with L. monocytogenes, with the highest incidence (53.01%) occurring in beef
meat. In the present study, most of the isolated strains belonged to serogroups IIB and IVB
(lineage I). The L. monocytogenes strain also contained monoA-B, prfA, plcA, plcB, mpl, hlyA,
actA, gtcA, dltA, Fri, flaA, InlA, InlC, InlJ, and iap genes. Biofilm formation was not determined
in the tested samples at pH 5.5 and different temperatures (4°C, 10°C, 25°C, and 37°C). However,
strong biofilm formation was observed in 6.45% (2/31) of the strains at pH 7.0 after 48 h incubation at 37°C, and in 3.22% (1/31) of the strains at pH 7.0 after 48 h incubation at 4°C and 10°C.
Pulsed-field gel electrophoresis (PFGE) results showed that L. monocytogenes isolates were
clonally related, and cross-contamination was present. In addition, PFGE results also revealed
that AscI had more distinguishing power than the ApaI restriction enzyme. These results indicate that L. monocytogenes detected from meat and meat contact surfaces in the slaughterhouse
pose a potential risk to public health.