The protective and therapeutic effect of phenoxy-2-methyl-2-propionic acid on experimental fatty liver in rats
TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.37, sa.6, ss.653-658, 2013 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 37 Sayı: 6
- Basım Tarihi: 2013
- Doi Numarası: 10.3906/vet-1212-28
- Dergi Adı: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.653-658
- Anahtar Kelimeler: Hepatic steatosis, rat, phenoxy-2-methyl-2-propionic acid, histopathologic examination, blood parameters, NONALCOHOLIC STEATOHEPATITIS, INJURY, RABBIT, STEATOSIS, DISEASE, MODEL
- İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır
Özet
In this study, the potential protective and therapeutic effect of phenoxy-2-methyl-2-propionic acid (PMPA) was investigated in rats with experimental hepatic steatosis induced by a carbon tetrachloride and paraffin liquid mixture (1:1). Forty Wistar albino rats were allocated to 4 groups with 10 rats in each. Group 1 was administered a saline solution; group 2 was administered the carbon tetrachloride and paraffin liquid mixture; group 3 was administered the carbon tetrachloride and paraffin liquid mixture and PMPA for 7 days; and in group 4, hepatic steatosis was created with the carbon tetrachloride and paraffin liquid mixture during the first 7 days, after which the rats were administered only PMPA during the last 7 days. All injections were done intraperitoneally. Blood samples were obtained from all rats and livers were extirpated before euthanasia. Blood parameters were found to be significantly different between the groups (P < 0.05). On histopathologic examination, grade 1 hepatic steatosis was observed in group 1; grade 3 hepatic steatosis and grade 2 portal fibrosis were observed in group 2; grade 2 hepatic fibrosis and grade 1 portal fibrosis were observed in group 3; and grade 1 hepatic steatosis was observed in group 4. Hepatic fibrosis was induced in the rats using a carbon tetrachloride and paraffin liquid mixture. Results indicated that PMPA has a limited protective effect and a superior therapeutic effect on hepatic steatosis.
Abstract: In this study, the potential protective and therapeutic effect of phenoxy-2-methyl-2-propionic acid (PMPA) was investigated
in rats with experimental hepatic steatosis induced by a carbon tetrachloride and paraffin liquid mixture (1:1). Forty Wistar albino
rats were allocated to 4 groups with 10 rats in each. Group 1 was administered a saline solution; group 2 was administered the carbon
tetrachloride and paraffin liquid mixture; group 3 was administered the carbon tetrachloride and paraffin liquid mixture and PMPA for
7 days; and in group 4, hepatic steatosis was created with the carbon tetrachloride and paraffin liquid mixture during the first 7 days,
after which the rats were administered only PMPA during the last 7 days. All injections were done intraperitoneally. Blood samples were
obtained from all rats and livers were extirpated before euthanasia. Blood parameters were found to be significantly different between
the groups (P < 0.05). On histopathologic examination, grade 1 hepatic steatosis was observed in group 1; grade 3 hepatic steatosis and
grade 2 portal fibrosis were observed in group 2; grade 2 hepatic fibrosis and grade 1 portal fibrosis were observed in group 3; and grade
1 hepatic steatosis was observed in group 4. Hepatic fibrosis was induced in the rats using a carbon tetrachloride and paraffin liquid
mixture. Results indicated that PMPA has a limited protective effect and a superior therapeutic effect on hepatic steatosis.
Key words: Hepatic steatosis, rat, phenoxy-2-methyl-2-propionic acid, histopathologic examination, blood parameters