Akademik Veri Yönetim Sistemi
International Ege Agriculture Congress, İzmir, Türkiye, 1 - 03 Kasım 2023, cilt.1, ss.54, (Özet Bildiri)
Hypericum perforatum L. (St. John’s wort), have been used for a long time
in folk medicine, is an herbaceous perennial plant growing in many regions in
Turkey. It has a significant antidepressant activity thanks to hypericin, one
of its bioactive ingredients.
The plant was harvested from Istanbul, Turkey. After cleaning, its aerial parts powdered and sieved. Considering to the literature and our previous studies, the solvent was chosen as water-ethanol mixture (50% v/v). The extractor system consists of four jacketed columns can work individually.
Extraction time is adjusted at the solvent flow rate
of 62.5 ml/min. For comparing to effects of temperature and column number, it
was studied at 35°C, 25°C, and 55°C while using one-, three-, and four-column
system, respectively.
It was found that while hypericin content were 0.076
g/g% dw with one column in 30 mins at 35°C, 0.403 g/g% dw with three columns in
113 mins at 25°C. After a process for 165 minutes, it was aimed to reach to maximum
total hypericin rate. For this, it was worked with four columns at 55°C to avoid
thermal degradation. At this condition, total hypericin content of the final product
was 2.34% g/g beside that the content of the third column was 0.51% g/g still.
It was seen that the findings proved the theorical expectations:
1. Hypericin content was highly influenced by solvent
flow rate.
2. Hypericin percentages increased with the process
time at first and then decreased.
3. The multi-column systems were efficient than the
one-column system.
4. It was understood that temperature is more
operative parameter than the column quantity for the aim.
With the battery-type extractor that was used, it was
seen that a semi-continuous production of hypericin-rich extract can be
possible at preferable and changeable process times and solvent flows without
thermal degradation.
Keywords: St. John’s Wort, Hypericum perforatum L., Extraction, Hypericin.