Detection of oseltamivir-resistant influenza A (H1N1) viruses with H274Y mutation during the 2007-2008 influenza season in Turkey Türkiye'de 2007-2008 influenza sezonunda oseltamivir dirençli influenza A (H1N1) virüslerinin H274Y mutasyonu ile saptanmasi
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.69, sa.1, ss.1-6, 2012 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 69 Sayı: 1
- Basım Tarihi: 2012
- Doi Numarası: 10.5505/turkhijyen.2012.23600
- Dergi Adı: Turk Hijyen ve Deneysel Biyoloji Dergisi
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.1-6
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır
Özet
Objective: In the beginning of the 2007-2008 influenza season in Northern Hemisphere, the frequency of influenza A (H1N1) viruses bearing a previously defined oseltamivir resistance increased dramatically. This is conferring to the amino acid exchange from histidine to tyrosine at position 274 (H274Y) in neuraminidase (NA) gene. The overall frequency of oseltamivir resistance in influenza A (H1N1) strains in Europe was reported as being 25%. Although it varied between countries, it was shown that the highest percentage was in Norway (67%), and the lowest was in Spain (2%). In this study, it was aimed to evaluate the oseltamivir resistance in influenza A H1N1 isolates from Turkey during the 2007-2008 influenza season. Method: During November 2007-May 2008, 20 samples were randomly selected between 73 influenza A (H1N1) positive samples detected with real-time RT-PCR in National Influenza Center (NIC) of Refik Saydam National Public Health Agency (RSNPHA). To detect such resistant viruses in Turkey, an RT-PCR assay was performed targeting amino acid position at 274 in NA gene of H1N1 influenza strain to investigate the presence or absence of histidine to tyrosine mutation.