A Novel Fluorescent Probe Based on Perylene Derivative for Hg2+ Ions and Biological Thiols and its Application in Live Cell imaging and Theoretical Calculations

Karuk Elmas Ş. N., Berk Gunay I., Karagoz A., Bostanci A., Sadi G., Yilmaz I.

ELECTROANALYSIS, cilt.32, sa.4, ss.775-780, 2020 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 4
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/elan.201900655
  • Dergi Adı: ELECTROANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Chimica, Communication Abstracts, Metadex, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.775-780
  • Anahtar Kelimeler: Perylene, Fluorescence Sensor, Mercury, Cysteine, Homocysteine, Glutathione, GLUTATHIONE, CYSTEINE, ELECTRODES, DIIMIDES, CARRIER, SENSOR
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır

Özet

The selective and sensitive detection of biothiols; cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) in aqueous solutions is of considerable importance because of their pivotal roles in maintaining the reducing environment in the cells. This study describes a strategy for the determination of biothiols based on the PDI/Met-Hg(2+)complex platform. We designed and fabricated methionine modified perylene diimide molecule as a selective sensing probe for Hg2+ ions in aqueous solutions (PDI/Met-Hg2+). The complex between perylene bisimide derivative (PDI/Met) and Hg2+ was investigated and it demonstrated turn-on fluorescence response for the detection of the biological thiols. Besides, PDI/Met displayed fluorescence quenching response in the presence of mercury ions and the emission intensity of PDI/Met-Hg2+ was recovered after transferring biothiols (Cys, Hcy, and GSH). Thus, PDI/Met could be utilized as a fluorescent chemosensor for the sequential recognition of mercury ions and biological thiols.