Research Information System
FEPS 2015 Joint Meeting of the FEBS and the Baltic Physiological Societies, Kaunas, Lithuania, 26 - 29 August 2015, vol.215, no.705, pp.88, (Full Text)
Aim: Alcoholism reduces bone turnover whereas estrogen deficiency results in elevated bone turnover. Reduction of the in bone turnover is likely to reduce the risk of osteoporosis in postmenopausal women because these individuals are losing bone because of elevated bone turnover. We investigated the effect of NO on bone metabolism in ovariectomized rats following chronic ethanol treatment.
Methods: The rats were divided into two groups. The first group received sham operation (SHAM) and the rats in Group II were ovariectomized (OVX). Five weeks after the SHAM and ovariectomy, the rats in each group were treated with ethanol for 4 months. After ethanol administration, the NOS inhibitor, L-NAME, was given for three weeks along with ethanol to the same rats. Serum IL-1beta, IL-6, TNF-alpha, NO, Ca, P, PTH, 25(OH)D3, ALP, bone- ALP, levels were measured in different stages of the experiment.
Results: IL-1beta, IL-6, TNFalpha and NO levels increased after ethanol administration in SHAM and OVX rats. The decrease in Ca was significant while the changes in P, PTH and 25(OH)D3 levels were not. ALP and bone- ALP levels were significantly decreased. In ovariectomized and SHAM rats, administration of L-NAME together with ethanol produced a significant increase in IL-1beta, IL-6 and TNFalpha levels. In this state, Ca and P levels were significantly increased; PTH and 25(OH)D3 levels were significantly decreased. Also, there was a significant decrease in, ALP, bone-ALP levels.
Conclusions: NO increase due to alcohol intake may function as a protective mechanism preventing bone resorption in cases of estrogen insufficiency.