Akademik Veri Yönetim Sistemi
Harran Üniversitesi Veteriner Fakültesi Dergisi, cilt.10, sa.1, ss.20-27, 2021 (TRDizin)
Foot and mouth disease (FMD) is one the most contagious diseases in cloven-hoofed animals and has a greatpotential for causing severe economic loss in susceptible animals. In the fight against FMD disease, inactive FMD vaccinesare widely used. This study was carried out for the detection of suitable BHK-21 cell lines in order to get virus suspensionscontaining excess amounts of 146S FMD virus serotypes O, A and Asia-1, for FMD vaccine production. For this purpose, inaddition to the BHK-21 An30 cell culture currently used in FMD vaccine production, BHK-21 An73 cell lines were passaged20 times. In every 5 passages, infective titers and 146S virus particle quantities of FMD virus serotypes O, A and Asia-1 weremeasured. Additionally, karyotype analysis was performed on both cells at the 20th passage levels and the tests revealedthat there was no significant change in the morphological features and chromosomal structures of the cells.It was determined that the infective titer and 146S virus particle quantities of serotypes O, A and Asia-1 of FMD virus werevery high in the BHK-21 An73 cell line than they are available in the BHK-21 An30 cell line. The increases of 146S virusparticle quantities of serotypes O, A and Asia-1 of FMD virus were statistically significant (p <0.05).As a result, high titer FMD 146S virus particles can be obtained in the vaccine production process by pre-determining theBHK-21 cell lines that are most sensitive to the FMD virus. Due to the increase in vaccine production capacity, it wasconcluded that it would make an important economic contribution to the producer.