Akademik Veri Yönetim Sistemi
5th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2022), Ankara, Türkiye, 23 - 25 Kasım 2022, ss.1664, (Özet Bildiri)
Gamma-radiation (RAD) causes changes in chemical balance of cells and impairs the reproductive potential. Although
chloroquine (CQ) is a drug used in treatment of malaria, chronic inflammatory diseases and of coronavirus (COVID-19).
It is known that apocynin (APO) strongly inhibits NADPH oxidases, and with this effect, it is a very important antioxidant
member to reduce oxidative stress. This study, it was aimed to evaluate the effects of RAD and CQ on testicular tissue
and to examine the effects of APO, which has antioxidant properties as a protective factor to reduce or prevent these
effects, on male fertility by biochemical and histological methods. In our study, male rats were divided into 8 groups;
(1)Control, (2)CQ, (3)APO, (4)RAD, (5)CQ+APO, (6)CQ+RAD, (7)APO+RAD and (8)CQ+APO+RAD. At the end of
the experiment, testicular tissues were taken and used for biochemical and histological experiments. Reduced glutathione
(GSH), lipid peroxidation (LPO), total antioxidant status (TAS), total oxidant status (TOS), reactive oxygen species
(ROS), oxidative stress index (OSI), nitric oxide (NO) levels and myeloperoxidase (MPO), lactate dehydrogenase (LDH),
sorbitol dehydrogenase (SDH) activities were determined. In CQ, RAD and CQ+RAD groups, LPO, TAS, TOS, ROS,
OSI, NO levels and MPO, LDH, SDH activities were found to be increased while GSH levels decreased. Administration
of APO reversed these changes. Tubules were categorized as normal, regressive, degenerative or atrophic as histological.
Regressive and degenerative seminiferous tubule structures and partial separations reflecting damage to the
spermatogenic series were observed in the testicular tissues of the RAD, CQ and CQ+RAD groups. Moreover,
intercellular space enlargements were noted. It was observed that healthy seminiferous tubule appearances were common
in the damaged groups treated with APO, similar to the control group. Results showed that APO had a protective effect
on the damage caused by CQ, RAD and CQ+RAD over biochemical and histological parameters.