Luteolin ameliorates colistin-induced nephrotoxicity in the rat models

Arslan B. Y., ARSLAN F., Erkalp K., Alagol A., Sevdi M. S., Yildiz G., ...Daha Fazla

RENAL FAILURE, cilt.38, sa.10, ss.1735-1740, 2016 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 10
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1080/0886022x.2016.1229995
  • Dergi Adı: RENAL FAILURE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1735-1740
  • Anahtar Kelimeler: Flavonoid, colistin, nephrotoxicity, CRITICALLY-ILL PATIENTS, ASCORBIC-ACID PROTECTS, POPULATION PHARMACOKINETICS, METHANESULFONATE, APOPTOSIS, KIDNEY, MICE, CARE, PATHWAYS, CELLS
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır

Özet

Introduction and aim: To study the protective, preventive effect of luteolin from colistin-induced nephrotoxicity.Material and method: Four different treatment options were tested on rats: colistin, luteolin, and a combination of colistin and luteolin, intraperitoneally as two doses a day, for seven days. Another group of rats were used as the control and treated with sterile saline. Serum creatinine levels were measured before and after treatment. Histological changes and colistin-induced apoptosis (Insitu BrdU-red DNA Fragmentation Assay Kit) of the renal tissues were examined after the scarification procedure.Results: In the Colistin Group, post-treatment creatinine levels were statistically higher than the pretreatment levels (p=.001). In the remaining groups, no significant changes were observed. Cells that undergo apoptosis were counted and it was shown that all groups except the colistin-treated group had a similar number of apoptotic cells, whereas the colistin-treated group had statistically higher number of apoptotic cells compared to other groups (p=.0001). Renal histological damage was also measured and the score of the colistin treated group was higher as compared to other groups.Conclusion: The results obtained from this study demonstrated us that luteolin was capable of preventing colistin-induced nephrotoxicity and that this effect was significant at histopathological level.