Molecular detection of endemic listeriosis and toxoplasmosis in shelter dogs in Istanbul, Turkey
International Journal of Veterinary Science, cilt.6, sa.1, ss.36-40, 2017 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 6 Sayı: 1
- Basım Tarihi: 2017
- Dergi Adı: International Journal of Veterinary Science
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.36-40
- Anahtar Kelimeler: Dogs, Listeria monocytogenes, Real Time PCR, Toxoplasma gondii
- İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır
Özet
Nowadays it is known that animal sourced infections may have serious threats against human health. In our study, we aimed to determine the molecular positivity of Listeriosis and Toxoplasmosis among shelter dogs in different animal shelters around Istanbul and to describe the role of dogs in the transmission of these zoonoses. Blood samples from 100 dogs were collected and Tag-Man probe based Real Time PCR (qPCR) analyses of the samples were conducted regarding to the high sensitivity and characteristics of this technique and the results were evaluated according to the gender and age of the dogs. According to our results, it is found that 12 dogs (12%) out of 100 are L. monocytogenes positive and 19 dogs (19%) are T. gondii positive. It is seen that seropositivity among the 0-2 ages group is high in both zoonoses and also according to gender L. monocytogenes is high among the females and T. gondii is high among the male dogs. We think that these results may be a serious risk for the people living in this city and optimal protective cautions should be taken. We estimate that our study will contribute the data about the prevalence of these zoonoses not only in our country but also all around the world.