Akademik Veri Yönetim Sistemi
TIAFT- 58th INTERNATIONAL ASSOCIATION OF FORENSIC TOXICOLOGSTS ANNUAL MEETING, 1 - 03 Şubat 2022, (Yayınlanmadı)
The vitreous humor (VH) is hydrophilic, transparent, post-mortem
ocular fluid, which is frequently preferred when blood and urine are
unavailable or contaminated during post-mortem forensic investigations. The
creatinine biomolecule, which takes place in VH generally uses as in postmortem
interval (PMI) estimations and ante-mortem kidney function assessments in the
field of forensic sciences. The analysis of creatinine biomolecule is crucial when
the PMI or antemortem kidney function are questioned. The aim of this study was
firstly the measurement of physicochemical properties of age-dependent prepared
artificial vitreous humor (A-VH) samples in order to obtain near-real VH and also
it was aimed to observe physicochemical changes of A-VH samples in the case of
blood contamination. A new method was developed
within creatinine-spiked A-VH samples by using both UV-visible
spectrophotometry and liquid chromatography-tandem mass spectrometry (LC-MS/MS)
to quantify creatinine concentration and to demonstrate the creatinine
concentration variations in relation to blood contamination. It was found that A-VH
samples which were prepared by phosphate buffered saline, showed a strong
similarity with natural VH. It was also observed that osmolality, pH, and
viscosity values of A-VH significantly changed when contaminated with blood. A
simple method was successfully developed by using UV-spectrophotometer with
good accuracy (≥85%) for determination of creatinine. The blood contamination
of A-VH samples caused variation of creatinine concentration. The new,
specific, sensitive, and accurate method was also developed for LC-MS/MS for
creatinine in A-VH and results were found linear, which ranged from 0,005-100
ng/mL with 0,999 correlation coefficient (r2). The detection and
quantification limit values were found 0,005 and 0,02 ng/mL, respectively. Creatinine
concentration of contaminated A-VH samples decreased significantly from 85% to 62%
recovery.