Effects of calcium silicate-based cements on odonto/osteogenic differentiation potential in mesenchymal stem cells

Sismanoglu S., Ercal P.

AUSTRALIAN ENDODONTIC JOURNAL, cilt.49, sa.1, ss.66-74, 2023 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49 Sayı: 1
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1111/aej.12615
  • Dergi Adı: AUSTRALIAN ENDODONTIC JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.66-74
  • Anahtar Kelimeler: calcium silicates, mesenchymal stem cells, odonto, osteogenic differentiation, pulp capping, vital pulp therapy, MINERAL TRIOXIDE AGGREGATE, PULPAL RESPONSES, BIODENTINE, MTA, CYTOTOXICITY, EXPRESSION
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır

Özet

The objective of this study was to evaluate the biological effects and odonto/osteogenic differentiation potential of Biodentine, NeoMTA Plus and TheraCal LC in tooth germ-derived stem cells (TGSCs). TGSCs were exposed to the material extracts. Biocompatibility was tested with MTS cell proliferation assay. Odonto/osteogenic differentiation was assessed with alkaline phosphatase (ALP) activity and mRNA gene expressions (RUNX2, DSPP and DMP-1). Scanning electronic microscopy/energy-dispersive X-ray (SEM/EDX) analysis and pH analysis were also performed for the materials. Data were evaluated using the one-way ANOVA and Tukey's tests. TGSCs remained viable after 7 days of incubation with all tested materials. Biodentine and NeoMTA Plus showed high ALP activity and increased expression of RUNX2, DSPP and DMP-1 compared to that of TheraCal LC. All materials can induce odonto/osteogenic differentiation of MSCs in various levels. Biocompatibility and odonto/osteogenic differentiation potential of Biodentine and NeoMTA Plus are similar and superior to that of TheraCal LC.