Effects of calcium silicate-based cements on odonto/osteogenic differentiation potential in mesenchymal stem cells
AUSTRALIAN ENDODONTIC JOURNAL, vol.49, no.1, pp.66-74, 2023 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 49 Issue: 1
- Publication Date: 2023
- Doi Number: 10.1111/aej.12615
- Journal Name: AUSTRALIAN ENDODONTIC JOURNAL
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, MEDLINE
- Page Numbers: pp.66-74
- Keywords: calcium silicates, mesenchymal stem cells, odonto, osteogenic differentiation, pulp capping, vital pulp therapy, MINERAL TRIOXIDE AGGREGATE, PULPAL RESPONSES, BIODENTINE, MTA, CYTOTOXICITY, EXPRESSION
- Istanbul University-Cerrahpasa Affiliated: No
Abstract
The objective of this study was to evaluate the biological effects and odonto/osteogenic differentiation potential of Biodentine, NeoMTA Plus and TheraCal LC in tooth germ-derived stem cells (TGSCs). TGSCs were exposed to the material extracts. Biocompatibility was tested with MTS cell proliferation assay. Odonto/osteogenic differentiation was assessed with alkaline phosphatase (ALP) activity and mRNA gene expressions (RUNX2, DSPP and DMP-1). Scanning electronic microscopy/energy-dispersive X-ray (SEM/EDX) analysis and pH analysis were also performed for the materials. Data were evaluated using the one-way ANOVA and Tukey's tests. TGSCs remained viable after 7 days of incubation with all tested materials. Biodentine and NeoMTA Plus showed high ALP activity and increased expression of RUNX2, DSPP and DMP-1 compared to that of TheraCal LC. All materials can induce odonto/osteogenic differentiation of MSCs in various levels. Biocompatibility and odonto/osteogenic differentiation potential of Biodentine and NeoMTA Plus are similar and superior to that of TheraCal LC.