Development of novel DNA-cuprac colorimetric probe for determination of hydroxyl radical damage and related antioxidant activity

Çekiç S. D., Uzunboy S., APAK M. R.

3rd IMEKOFOODS Conference: Metrology Promoting Harmonization and Standardization in Food and Nutrition, Thessaloniki, Yunanistan, 1 - 04 Ekim 2017, ss.122-125, (Tam Metin Bildiri) identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Cilt numarası:
  • Basıldığı Şehir: Thessaloniki
  • Basıldığı Ülke: Yunanistan
  • Sayfa Sayıları: ss.122-125
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır

Özet

Copyright� (2017) by the International Measurement Confederation (IMEKO). All rights reserved.An unbalance between reactive oxygen species and antioxidants in favour of oxidants may cause health issues. In the study DNA was used as probe material to measure oxidative damage caused by hydroxyl radical (�OH) and potential protective effects of certain water soluble antioxidants.�OH was produced by Fenton method and a modified colorimetric CUPRAC method was developed. The absorbance difference originated from the fact DNA degradation products but not the original DNA was responsive to the CUPRAC reagent.