Immunofluorescent Visualization of H3K27ac and H3K27me3 Histone Modifications in Normal and Cancer Chromosomes
15th European Cytogenomics Conference, Leuven, Belçika, 29 Haziran - 01 Temmuz 2025, sa.56, ss.84, (Özet Bildiri)
- Yayın Türü: Bildiri / Özet Bildiri
- Basıldığı Şehir: Leuven
- Basıldığı Ülke: Belçika
- Sayfa Sayıları: ss.84
- İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet
Özet
Epigenome, the general epigenetic state of an organism, is as important as the genome in normal development. Epigenetics is the study of heritable changes in gene expression that occur through changes in chromatin structure and organization rather than in the DNA sequence. Cancer epigenetics studies today are almost exclusively at the molecular level. There is currently no field of “cancer cytoepigenetics” that corresponds to cancer cytogenetics, which is a very important branch of cancer genetics. If epigenetic changes can be demonstrated on chromosomes, it may be possible to monitor changes in epigenetic regulation, just as it is possible to monitor karyotype changes in cells during disease progression. The introduction of such a technique would be of great benefit in clinical applications. Chemical modifications in histone proteins can induce the formation of a permissive euchromatin or a repressive heterochromatin state. The aim of this study was to examine histone modifications that form permissive and repressive chromatin states regarding gene expression in normal and cancer chromosomes by immunofluorescence methods and to determine whether there is a difference between them in this respect. The study included healthy individuals to obtain normal chromosomes and K562 cell-line for cancer chromosomes. Immunofluorescent staining was performed using specific antibodies for permissive chromatin marker H3K27ac and repressive chromatin marker H3K27me3 to chromosome preparations from healthy subjects and K562 cell-line. The band patterns obtained in both sample types showed that both modifications were concentrated in gene-rich regions which roughly corresponds to light G bands. For both histone modifications examined, no significant difference was observed in immunofluorescent band patterns of chromosomes (chromosomes 1, 12 and 19) that could be evaluated in both normal and cancer samples.