Isolation of<i> Aeromonas</i><i> bestiarum</i> in rainbow trout<i> (Oncorhynchus</i><i> mykiss)</i> and determination of antibacterial resistance profile
WIENER TIERARZTLICHE MONATSSCHRIFT, cilt.112, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 112
- Basım Tarihi: 2025
- Dergi Adı: WIENER TIERARZTLICHE MONATSSCHRIFT
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE
- İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır
Özet
Aeromonas species are microorganisms that can cause clinically significant diseases in fish and humans. We have isolated bacterial pathogens from samples of fish infected with Aeromonas species, determined their antibiotic susceptibility profiles and investigated their antibiotic resistance. We examined ten rainbow trout with symptoms of the disease. After necropsy, we isolated and identified bacteria from the lesions in the tissues and organs. We performed culture confirmation and species identification using Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). We determined the phenotypic antibiotic susceptibility of Aeromonas isolates by the Kirby-Bauer disk diffusion method and evaluated the results according to the standards of the Clinical and Laboratory Standards Institute (CLSI). For phenotypic resistance, we used commercially prepared ready-to-use antibiotic discs of the antibacterial groups tetracyclines, carbapenems, penicillins, amoxicillin/clavulanic acid, folic acid synthesis inhibitors, quinolones, aminoglycosides, amphenicols and macrolides. We also analysed the expression of the antibacterial resistance genes blaACC, sul1, tetA and hfq. Aeromonas bestiarum isolates exhibited phenotypic resistance to ampicillin, penicillin, amoxicillin and erythromycin. Comparative genotypic analysis of the resistance genes blaACC, sul1 and tetA with the reference strain revealed the expression of gene seg-ments. We conclude that Aeromonas bestiarum agents cause severe infections in rainbow trout. We recom-mend testing diseased fish for Aeromonas and selecting the appropriate antibacterial agent using antibiograms.