Effect of Moringa oleifera Lam. Extract on Antioxidant Markers in Muscle Tissue of Valproate Administered Rats

Ertik O., Magaji U. F., Saçan Ö., Yanardağ R.

The 4th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2021), Ankara, Türkiye, 24 - 26 Kasım 2021, ss.1141, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.1141
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet

Özet

Valproic acid (VPA) is commonly used in the treatment of epilepsy, but serious complications may occur in some patients, including hemorrhagic pancreatitis, coagulopathies, bone marrow suppression, VPA-induced hepatotoxicity and encephalopathy. Moringa oleifera leaves are widely used in many tropical countries, as a good source of antioxidant compounds such as ascorbic acid, flavonoids and phenolics. Studies on the biological effects of M. oleifera have shown that the plant has potential antitumor, anti-inflammatory, antiulcer, antispasmodic, diuretic, antihypertensive, antidiabetic and hepatoprotective effects. The current study was designed to investigate the effects of moringa leaves extract (70% ethanol) on antioxidant system against VPA-induced oxidative damage in muscle tissues of rats. In the study, female Sprague Dawley rats were randomly divided into four groups. Group I: control group (n=8); Group II: animals given only 70% ethanol extract of moringa leaves for 15 days (0.3 g/kg b.w. /day) (n=8); Group III: animals that received only sodium valproate for 15 days (0.5 g/kg b.w. /day) (n=15); Group IV: animals administered with similar dose of sodium valproate+moringa extract for 15 days (n=12). Moringa extract and sodium valproate were administered orally. Group I was orally given a similar dose of physiologic saline. At the end of the experiment, muscle tissues were taken. Total antioxidant (TAS), total oxidant (TOS), reactive oxygen (ROS), oxidative stress index (OSI), nitric oxide (NO), sialic acid (SA), glutathione (GSH), lipid peroxidation (LPO) levels, lactate dehydrogenase (LDH) and myeloperoxidase (MPO) activities were determined in muscle tissues. In VPA groups, TOS, ROS, OSI, NO, SA, LPO levels, LDH and MPO activities were found to be raised, while TAS and GSH levels diminished. Administration of M. oleifera extract reversed these changes. These results showed that M. oleifera leaves extract had a protective effect on muscle tissues against VPA damage.