Akademik Veri Yönetim Sistemi
7th ASIA PACIFIC International Modern Sciences Congress, Jakarta, Endonezya, 4 - 05 Kasım 2022, ss.453, (Özet Bildiri)
Diabetes is a metabolic and chronic disease that affects the metabolism of different tissues. It can be caused by genetic factors, lifestyles, and dietary habits. In diabetes, oxidative stress can occur in metabolic disorder and it effects negatively. The antioxidants are of great importance in reducing or completely stopping the harmful effects of these negative effects on the tissues. In the present study, we aimed to determine of combined effects of lipoic acid, vitamin E, and selenium in the kidneys of diabetic mice. For this experiment, The Balb/c mice were used and divided into five groups; citrate buffer (C), the solvents of the antioxidants (SA), combined the antioxidants (A) [α-lipoic acid (50 mg/kg), vitamin E (100 mg/kg) and selenium (0.25 mg/kg)], streptozotocin (D) [as five consecutive daily doses of 40 mg/kg for diabetes], combined with the antioxidants and streptozotocin (A+D). At the end of 30 days of this process, the mice were sacrificed by cervical dislocation and kidney tissues were taken for biochemical and morphological analyses. The kidney tissue samples were homogenized in cold physiological saline (10% (w/v)) and the homogenates were centrifuged at 10.000g for 10 minutes. After that, the homogenates were used for analyses of biochemical parameters such as the level of reactive oxygen species (ROS), total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI) and the activities of lactate dehydrogenase (LDH), adenosine deaminase (ADA), carbonic anhydrase (CA) and arginase. The biochemical and morphological results revealed that the administration of combined antioxidants (α-lipoic acid, vitamin E, and selenium) to diabetic mice has a partly protective effect on the kidney tissue.