Akademik Veri Yönetim Sistemi
3rd Internatinal Cancer and Ion Channels Congress, İstanbul, Türkiye, İstanbul, Türkiye, 16 - 18 Eylül 2021, ss.60, (Özet Bildiri)
Curcumin molecule, which is the active component of turmeric spice, is one of the most important members of Chinese traditional medicine for centuries. Curcumin which is isolated from Curcuma Longa has different pharmacological effects such as antimicrobial, antifungal, antioxidant, antiinflammatory, antitumor, anti-HIV, anti-Alzheimer's, anti-diabetic. It can suppress tumor initiation, progression and metastasis. Lung cancer, which causes the death of 1.8 million people every year, is one of the most important cancer types that threaten human life. Curcumin has been reported to affect different molecular pathways such as vascular endothelial growth factors, nuclear factor-κB (NF-κB), mammalian target of rapamycin, PI3/Akt, microRNAs and long noncoding RNAs in the treatment of lung cancer. DNA methylation is the reaction of covalent attachment of a methyl group from the 5-carbon of cytosine to the structure of a CpG dinucleotide, which plays a role in the carcinogenic process, and alters cell functions by altering gene expression. The presence of hypomethylation in lung cancer cells has been reported in the literature. In this study, we aimed to investigate the effects of curcumin-benzoquinone derivative which is synthesized by our group/ applied for patent (Patent No: 2020/22499) and previously determined to have anti-proliferative effects on breast cancer cell lines (MDA-MB-231 and MCF7) on A549 lung cancer cell line. Method: Curcumin-benzoquinone derivative (1,5 and 10 µg/ml) was performed to both the A549 lung cancer and the HUVEC cell line that we used as a control, and 3-(4,5-Dimethylthiazol-2-yl)-2,5 -Diphenyltetrazolium Bromide (MTT) and global DNA methylation analyzes by ELISA were performed. Concentrations was determined as a results of MTT assay. It was observed that cell proliferation was significantly reduced in A549 cell lines treated with curcumin-benzoquinone derivative molecule compared to the control group. In addition, a percentage increase was observed in the global methylation analyzes. While the percent methylation was 1.625 in the control A549 cell line where the synthesized molecule was not applied, the percent methylation values were found to be 1.741, 2.812 and 1.946 in the cell lines treated with 1.5 and 10 µg/ml curcumin-benzoquinone derivative, respectively. As a result, the newly synthesized curcuminbenzoquinone derivative molecule has an antiproliferative effect by reducing hypomethylation in the A549 lung cancer cell line, suggesting that it may be a drug candidate molecule for the treatment of lung cancer.
Keywords: Curcumin, benzoquinone, A549 lung
cancer, antitumor activity, MTT, Global methylation