Akademik Veri Yönetim Sistemi
Turkish Journal of Sports Medicine, cilt.55, sa.2, ss.131-137, 2020 (TRDizin)
ABSTRACT Objective: The aim of this study was to investigate
the changes in certain oxidative stress and antioxidant system parameters in
response to acute exhaustive exercise (AEE) applied to diabetes-induced rats.
Methods: 16 male Wistar rats were divided diabetes (n=8) and control (n=8)
groups. For diabetes induction, 65 mg/kg of streptozotocin (STZ) administered
to rats intraperitoneally. On the fourth day of STZ administiration, blood
glucose level was measured and level of above 180 mg/dL was considered as
diabetes mellitus (DM). AEE procedure was applied to rats within the same day
that they were considered as diabetes. After the exercise protocol, blood
samples were collected for biochemical analyses and serum samples were obtained
by centrifugation. 3-nitrotyrosine, LPx, protein carbonyl, SOD and GSH, GSH-Px
levels were analyzed by using Enzyme-linked immunosorbent assay. Results: After
the exercise, both groups had decreased blood glucose levels compared to
pre-exercise levels. There was a significant increase in lactate levels of both
groups, compared to pre-exercise levels. In terms of distance run, control
group ran approximately two times more distance than the diabetes group. When
we compared oxidative stress parameters; Lipid peroxide, 3-nitrotyrosine, and
protein carbonyl levels were significantly higher in the diabetes group.
Antioxidant parameters; glutathione and glutathione peroxidase were
significantly higher in the diabetes group. As for superoxide dismutase levels,
it was slightly decreased in the diabetes group, but did not reach statistical
significance. Conclusions: AEE triggers both oxidant and antioxidant systems in
diabetic rats. Keywords: Diabetes Mellitus, acute exhaustive exercise,
oxidative stress, antioxidants