Molecular detection and prevalence of feline hemotropic mycoplasmas in Istanbul, Turkey

ÇETİNKAYA H., Haktanir D., Arun S., Vurusaner C.

ACTA PARASITOLOGICA, cilt.61, sa.1, ss.165-171, 2016 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 61 Sayı: 1
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1515/ap-2016-0022
  • Dergi Adı: ACTA PARASITOLOGICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.165-171
  • Anahtar Kelimeler: Cat, Mycoplasma sp., PCR, RFLP, Turkey, REAL-TIME PCR, POLYMERASE-CHAIN-REACTION, RISK-FACTOR ANALYSIS, ARTHROPOD-BORNE PATHOGENS, HAEMOPLASMA INFECTION, HAEMOBARTONELLA-FELIS, HAEMOTROPIC MYCOPLASMAS, DOMESTIC CATS, TOXOPLASMA-GONDII, RICKETTSIA-FELIS
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Hayır

Özet

The aim of this study was to investigate Mycoplasma spp. species in blood samples of the domestic cats from the province of Istanbul, Turkey. Three hundred eighty four blood samples of client-owned cats were used for the identification of Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMhm) and Candidatus Mycoplasma turicensis (CMt) by Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) assays. Out of 384 blood samples, 74 (19.3%) were positive for one of Mycoplasma species. The total prevalence of Mhf, CMhm and CMt infections was 9.9%, 17.7% and 0.8% respectively. The most common species was CMhm. Co-infections were mostly with Mhf/CMhm and the frequency was 8.1%. Two cats were infected with three species. The current study was the first molecular prevalence study of hemotropic mycoplasmas in Istanbul, reporting the presence of CMt for the first time in Turkey. Prevalence of feline mycoplasma was notably high in Istanbul and PCR assay could be preferred rather than the microscopic examination for the diagnosis.