Comparative study of follicular fluid and cultured granulosa cell steroid production
10th World Congress of In Vitro Fertilization and Assisted Reproduction, Vancouver, Kanada, 24 - 28 Mayıs 1997, ss.365-368, (Tam Metin Bildiri)
- Yayın Türü: Bildiri / Tam Metin Bildiri
- Basıldığı Şehir: Vancouver
- Basıldığı Ülke: Kanada
- Sayfa Sayıları: ss.365-368
- Anahtar Kelimeler: granulosa cell, in vitro fertilization, steroid production
- İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet
Özet
To determine the steroidogenic capacity of follicular and cultured granulosa cells, estradiol and progesterone productions were studied in 10 fertilized and 10 nonfertilized IVF-ET cases. After separation of granulosa cells, they were cultured in EBSS within 24 hours. Follicular fluid obtained from follicles with fertilized oocytes contained higher levels of estradiol (E2), progesterone (P) and P/E2 ratio although the differences were statistically nonsignificant. Cultured granulosa cells demonstrated higher numbers per mililiter in fertilized oocyte group in comparison with the nonfertilized ones (p<0.05). However, nonfertilized group showed higher estradiol and progesterone production per 10 million granulosa cells (p<0.05) while P/E2 production rate of cultured granulosa cells was higher than the P/E2 ratio of fertilized group (p<0.05). These results suggest that preovulatory granulosa cell steroid production in favor of progesterone may deteriorate fertilization.