Akademik Veri Yönetim Sistemi
Microchemical Journal, cilt.225, 2026 (SCI-Expanded, Scopus)
Water-soluble conjugated polyelectrolytes (CPEs) are polymers that display unique optical and electronic properties suitable for fluorescence sensing and detection of biological-related molecules. In this study, we present the synthesis of a novel cationic conjugated polyelectrolyte (P-P1) with phosphonium functionality and describe the sensing properties of this polymer towards the chemosensory detection of pyrophosphate anion and nucleotides. The sensing mechanism of the polymer follows the aggregation-induced quenching (AIQ) phenomenon driven by electrostatic interactions between P-P1 and PPi/nucleotide molecules. The study incorporates the evaluation of the photophysical properties through steady-state spectroscopy and assessment of the amplified fluorescence quenching response of P-P1 in presence of different nucleotides. A multivariate data analysis methodology served to evaluate spectral shape variations of the collected emission data using Principal Component Analysis (PCA), Linear Discrimination Analysis (LDA) and Soft Independent Modelling of Class Analogy (SIMCA) for the classification of 13 different nucleotides. By analyzing the normalized emission intensities of selected wavelength ranges of P-P1, this methodology achieved near 100% accuracy for efficient classification of nucleotides and DNA oligonucleotides.