Akademik Veri Yönetim Sistemi
Testing the Waters 6, Oxford, İngiltere, 26 - 27 Haziran 2023, (Yayınlanmadı)
Monitoring
the consumption and trends of New Psychoactive Substances (NPS) is an issue
that countries are closely interested in due to its effect on public health,
safety and economy. However, determining the substance trends and consumption
amounts in a society can only be possible with a holistic approach. From this
point of view, the wastewater-based epidemiology (WBE) approach, whose ability to
determine the consumption and trends of legal/illegal substances in societies
has been proven internationally, is seen as an important indicator for the
determination of NPS consumption. In WBE studies carried out in the literature
so far, the research on synthetic cannabinoid group substances is limited due
to the rapid metabolization, the presence of common metabolites, the lack of
widespread consumption information and/or the lack of reference standards. As a
result of these challenges and higher consumption rates of stimulant groups,
studies are rather focused on synthetic cathinone, tryptamine, piperazine and
phenethylamine derivative stimulants. However, considering synthetic
cannabinoids are the most seized NPS group in Turkey, it has become vital to
develop synthetic cannabinoid-oriented analytical method covering other NPS
groups as well in order to achieve anonymous, evidence-based data on the
consumption of NPS [1]. In light of this view, a comprehensive analysis method was
aimed to develop for the first time in Turkey in order to simultaneously detect
the consumption of 26 NPS including 19 synthetic cannabinoids, 6 synthetic
cathinones and 1 phencyclidine type substance via wastewater. All the
substances were chosen according to national seizure data or among substances
suspected to be used by law enforcement. Selectivity, linearity, limit of
detection (LOD), limit of quantitation (LOQ), accuracy, matrix effect and
robustness studies were conducted for all targeted NPS. The linear range of the
analytical method varied in between 0.20-100.00 ng/L; while LOQ values in
wastewater ranged between 0.50-30.00 ng/L. A solid phase extraction procedure
has been developed with the Oasis HLB cartridge, which has a recovery
performance of 80.16%-119.98% for all analytes. With this method, it has become
possible to perform the qualitative and quantitative analysis of a total of 26
NPS simultaneously. The validated method was also applied to authentic samples
to verify the suitability of the method. To the best of our knowledge, this
study is the first in the field of monitoring NPS from wastewater in Turkey; as
for Cumyl-4-CN-B7AICA, ADB-FUBINACA, AM-2201-4-hydroxypentyl metabolite,
4F-MDMB-BINACA-N-(4-hydroxybutyl) metabolite, 4F-MDMB-BINACA-butanoic acid
metabolite, 5F-ADB-PINACA, 5F-BZO-POXIZID and BZO-HEXOXIZID substances, the
first study in the literature regarding to their application to wastewater.