Akademik Veri Yönetim Sistemi
Erciyes Tip Dergisi, cilt.30, sa.3, ss.132-137, 2008 (SCI-Expanded, ESCI, Scopus, TRDizin)
Purpose: Glutathione S-transferase M1 (GSTM1) enzyme has been suggested to serve as a steroid-binding protein by its ability to bind to testosterone and estradiol, two important hormones in seminiferous tubule that are essential for spermatogenesis. We investigated whether GSTM1 polymorphism was associated with blood and seminal plasma levels of testosterone and estradiol in subfertile and control subjects. Material and Methods: The levels of total estradiol and testosterone were measured an electrochemiluminescence immunoassay in serum and seminal plasma from 103 individuals including 62 subfertile patients. GSTM1 polymorphism was examined using polymerase chain reaction. Results: The estradiol and testosterone levels in seminal plasma were not significantly differant in the control and subfertile subjects. No role for GSTM1 enzyme as a steroid-binding protein seemed likely as there was also no significant difference in seminal plasma estradiol and testosterone levels according to GSTM1 genotype. Using Spearman rank correlation analysis, significant positive correlations were found between seminal estradiol and serum estradiol in infertile males, and between seminal testosterone and serum testosterone in fertile males, independent of GSTM1 genotype. Conclusion: The results suggest that GSTM1 polymorphism is not a genetic risk factor for seminal estradiol and testosterone levels in infertile males.