The Effect of Microplastic and Cadmium Exposure on the Enzyme Activities of Mytilus galloprovincialis (Lamarck, 1819)

Dağsuyu E., Türkyılmaz İ. B., Can Tunçelli İ., Tunçelli G., Erkan Özden N., Yanardağ R.

4th International Eurasian Conference on Science, Engineering and Technology (EurasianSciEnTech 2022), Ankara, Türkiye, 14 - 16 Aralık 2022, ss.27, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.27
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet

Özet

Marine environments are exposed to massive pressure from microplastics (<5 mm). The effects of environmental microplastics (MPs) combined with other pollutants are still poorly investigated. One of the most widespread and hazardous metal pollutants found in the environment is cadmium (Cd). There is a high likelihood that Cd and MPs can co-occur in aquatic environments. In this study, the effects of different microplastics and cadmium exposure on enzyme activities in Mediterranean mussels, which can be found along the coastlines in the Mediterranean region, are examined. The mussels were divided into eight groups. The exposure period lasted for 7 days. Exposure was renewed every third day. Eight treatments with two replicates exposure period were used: Control group (C): full artificial seawater was given with no stressors, polystyrene (PS, 2 mg L−1), polypropylene (PP, 2 mg L−1), polyethylene terephthalate (PET, 2 mg L−1), cadmium chloride (CdCl2, 30 µg L−1 Cd2+), PS+Cd (2 mg L−1 and 30 µg L−1 Cd2+), PP+Cd (2 mg L−1 and 30 µg L-1 Cd2+) and PET+Cd (2 mg L −1 and 30 µg L−1 Cd2+) groups were exposed to them for 3 and 7 days. At the end of the study, all the mussels were collected and mantle tissues were taken and homogenized in physiological saline. The glutathione (GSH), lipid peroxidation (LPO) and reactive oxygen species (ROS) levels and acetylcholinesterase (AChE), alanine transaminase (ALT) and lactate dehydrogenase (LDH) activities were determined in the homogenates. According to the results, the experimental groups’ GSH levels and AChE activities decreased compared to the control group on the 3rd and 7th days. The experimental groups’ LPO and ROS levels and ALT and LDH activities increased compared to the control group on the 3rd and 7th days. In this study, it was determined that MP and Cd exposure affected the mussel physiology and showed significant changes in enzyme activities.