Alterations in expression of phosphatase and tensin homologous (PTEN) gene analyzed in the bronchial lavage samples of non-small cell lung cancer patients
ANNALS OF CLINICAL AND ANALYTICAL MEDICINE, cilt.15, sa.1, ss.11-15, 2024 (ESCI)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 15 Sayı: 1
- Basım Tarihi: 2024
- Dergi Adı: ANNALS OF CLINICAL AND ANALYTICAL MEDICINE
- Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI)
- Sayfa Sayıları: ss.11-15
- İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet
Özet
Objective: The aim was to analyze the expression of Phosphatase and Tensin Homologous gene (PTEN) gene by interphase fluorescence in situ hybridization (iFISH), and localization and immunoreactivity of PTEN by immunocytochemistry method in bronchoalveolar lavage (BAL) fluid of patients with non-small cell lung cancer (NSCLC).
Methods: BAL samples collected from 30 patients diagnosed with NSCLC and from 10 control subjects without any malignancy were included. iFISH was performed with 10q23. 3 PTEN DNA dual color FISH probe, analyzing multiple cells including normal cell (2G2R), biallelic deleted cell (2G), monoallelic deleted cell (2G1R), monosomic cell (1G1R) and atypical signals. Immunocytochemistry was based on Streptavidin-Biotin-Peroxidase method and PTEN levels were semi-quantitatively scored.
Results: Percentage of cells with 2G/2R was 86.8% which was, significantly lower than that of control group (p< 0.0001). Loss of PTEN gene was 6.43% among all patients. The Percentage of cells with monoallelic deletion (p= 0.0246) and atypical signal (p= 0.0001) and percentage of monosomic cells (p= 0.0001) were significantly higher in patients compared to controls. PTEN immunopositivity was significantly increased in patients compared to controls (p< 0.0001).
Conclusion: The signal characteristics of cells can alter among NSCLC patients. The increased abnormal signals and mutational cells in BAL samples of NSCLC patients suggest that the investigation of PTEN expression in BAL by using molecular cytogenetic methods may guide the clinicians in differential diagnosis and therapeutic management of NSCLC …