Akademik Veri Yönetim Sistemi
CLINICAL RHEUMATOLOGY, cilt.44, sa.10, ss.4353-4365, 2025 (SCI-Expanded, Scopus)
Objective This study aimed to evaluate the therapeutic effects of extra virgin olive oil (EVOO), known for its antioxidant and cytoprotective properties, in a monosodium iodoacetate (MIA)-induced rat model of osteoarthritis (OA). Methods Twenty-one male Wistar rats were randomly divided into three groups: Control, OA, and EVOO-treated. OA was induced via intra-articular injection of MIA. The EVOO Group received a diet supplemented with EVOO for 21 days post-induction. Histopathological, immunohistochemical, biochemical, and radiological analyses were performed to evaluate cartilage damage, inflammation, apoptosis and oxidative stress. Results Histopathological evaluation revealed moderate cartilage destruction and synovitis in the OA Group, which were reduced in the EVOO-treated Group. Safranin-O staining confirmed higher proteoglycan preservation with EVOO supplementation. Immunohistochemistry demonstrated low intensity of oxidized low-density lipoprotein (ox-LDL) in the EVOO-treated Group compared to the OA Group. TUNEL staining indicated a significant reduction in chondrocyte apoptosis in EVOO-fed rats. Biochemically, ox-LDL and other oxidative stress markers, including total oxidative status (TOS) and oxidative stress index (OSI), were significantly reduced in the EVOO Group compared to the OA Group (p < 0.05). In contrast, the antioxidant capacity (TAC) level was significantly higher in the EVOO Group (p < 0.05). A positive correlation was observed between ox-LDL and TOS (p = 0.005). Conclusions Our findings suggest that EVOO may contribute to cartilage preservation and reduce synovitis in rats with MIA-induced OA. Additionally, EVOO exhibits significant antioxidant effects by reducing ox-LDL and oxidative stress markers and enhancing antioxidant capacity. These results may highlight EVOO as a potential adjuvant therapy for managing OA.