Cryoprotective effects of cholesterol-loaded cyclodextrin and iodixanol on ram sperm: assessment of post-thaw quality and in vitro fertilization outcomes

Arıcı R., Eser A., Yağcıoğlu S., Demir K., Pabuccuoğlu S., Ak K., ...Daha Fazla

BMC VETERINARY RESEARCH, cilt.23, ss.1-30, 2026 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23
  • Basım Tarihi: 2026
  • Doi Numarası: 10.1186/s12917-026-05533-5
  • Dergi Adı: BMC VETERINARY RESEARCH
  • Derginin Tarandığı İndeksler: Scopus, Science Citation Index Expanded (SCI-EXPANDED), EMBASE, MEDLINE, Directory of Open Access Journals
  • Sayfa Sayıları: ss.1-30
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet

Özet

This study aimed to investigate the effects of cholesterol-loaded cyclodextrin (CLC) and iodixanol, added individually or in combination to a ram semen extender, on post-thaw sperm quality, in vitro fertilization outcomes, and embryo development. Semen samples were divided into four experimental groups: Control, CLC (2 mg CLC/120 × 106 spermatozoa), Iodixanol (5%, v/v), and CLC + Iodixanol. All samples were diluted with a tris-based egg yolk extender and cryopreserved. Following thawing, sperm motility and kinetic parameters were assessed using a computer-assisted sperm analysis (CASA) system. A thermal stress test was applied, followed by re-evaluation via CASA. Sperm viability and acrosomal integrity were determined using fluorescence microscopy, and plasma membrane functional integrity was evaluated via the hypo-osmotic swelling test (HOST). Post-thaw semen samples from all groups were used for in vitro fertilization to assess cleavage and embryo development rates.