Akademik Veri Yönetim Sistemi
5th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2022), Ankara, Türkiye, 23 - 25 Kasım 2022, ss.52, (Özet Bildiri)
The thioredoxin system consists of thioredoxin reductase (E.C 1.6.4.5; TrxR), thioredoxin (Trx) and reduced
nicotinamide adenine dinucleotide phosphate (NADPH). TrxR is a flavin adenine dinucleotide (FAD)-containing
NADPH-dependent disulfide reductase. Spirulina platensis (SP) is a phototrophic, filamentous, and multicellular bluegreen microalga, belonging to the class of Cyanobacteria. Spirulina with its high nutritional value has been consumed as
food. The importance of the system, which has a wide variety of functions for the cell, is due to the role of TrxR in
protecting against oxidant damage, cell growth and transformation, p53 activity, and recycling of ascorbate from its
oxidized form. In this study, the in vitro effects of some vitamins and drugs on TrxR purified from commercial SP tablets
were investigated. For this purpose, the TrxR was purified from commercial SP tablets. Purification processes were
performed in two steps: preparation of the homogenate and 2',5'-ADP Sepharose 4B affinity chromatography. The effects
of vitamins and various drugs on the purified TrxR activity were investigated. The IC50 value, the amount of substance
required for the enzyme to have a 50% inhibition effect, was calculated. The inhibition types of compounds were
determined with the help of Lineweaver–Burk graphs. As a result of these experiments, it was found that the vitamins
and drugs had inhibitory effects on the TrxR enzyme.