Research Information System
7th European Congress of Pharmacology, İstanbul, Turkey, 26 - 30 June 2016, pp.313, (Full Text)
We aimed to study pitavastatin’s effects on Nuclear Factor-kappa B (NF-κB) and adhesion molecules in human saphenous vein graft endothelial culture indicating its pleotropic properties. Low-dose (0.1 μM/L) and high-dose (1 μM/L) pitavastatin calcium were administered as a frontline therapy in human saphenous endothelial cell culture, followed by induction of inflammation by TNF-alpha and determination of mRNA level alterations of VCAM-1, ICAM1, and NF-κB genes of endothelial cells using the qRT-PCR method. Additionally, immunofluorescence method was used to show the expression of NF-κB and ICAM-1. Finally, LDH levels were determined by the ELISA method to quantify cytotoxicity. ICAM-1 mRNA expression in the low-dose pitavastatin+TNF-alpha group was significantly higher than that in the TNF-alpha group and significantly lower than that in the high-dose pitavastatin+TNF-alpha group (for all comparisons, P=0.001). VCAM-1 mRNA expression in the low-dose pitavastatin+TNF-alpha group was higher than those of the TNF-alpha and high-dose pitavastatin+TNF-alpha groups (for all comparisons, P = 0.001). VCAM-1 mRNA expression was significantly lower in the high-dose pitavastatin+TNF-alpha group than the TNF-alpha group. The low-dose pitavastatin+TNF-alpha group had a similar NF-κB mRNA expression with TNF-alpha and high-dose pitavastatin+TNF-alpha groups. Pitavastatin increases ICAM-1 mRNA expression in saphenous vein endothelial cells. However, it increases VCAM-1 in low dose but decreases it in high dose. Furthermore, the effect of pitavastatin on adhesion molecules appears independent of NF-κB. Novel studies are needed in this field.