Akademik Veri Yönetim Sistemi
Middle East Fertility Society Journal, cilt.6, sa.1, ss.19-25, 2001 (Scopus)
Objective: To test the predictability value of combined computer assisted semen analysis (CASA) and hypoosmotic swelling (HOS) on fertilization in IVF. Several tests have been proposed to predict the fertile outcome of in vitro fertilization. Many of these tests are related to sperm function. HOS test and CASA measure sperm function though these functions may be independent from each other. Combining the two tests we can predict the fertile outcome of IVF more exactly than using the tests separately. Materials and methods: We investigated the two tests in 43 infertile couples undergoing IVF treatment. HOS test-and CASA was performed in 43 patients. Conventional HOS tests and subtypes of HOS test were investigated as well as the most disciminatory value of HOS was determined. CASA was performed by using capacitated sperm and 11 parameters were investigated, besides, cutoff values for detection of fertilization of each parameter were determined. Results: HOS test and HOS A and HOS C subtypes significantly altered in fertilizated cases as compared to non fertilized cases (p=0.007, p=0.026 and p=0.029 respectively). CASA also showed some differences in fertilized cases. ALH, VCL, VAP, hyperactivity and motility were significanly higher in fertilized cases than non fertilized cases (p<0.001, p=0.004, p=0.27, p=0.001 and p=0.027 respectively). ALH, hyperactivity, motility (p<0.001), and VCL correlated with fertilization (p<0.02) as well as the HOS test (p<0.01). We observed that conventional HOS test limits were not sufficient to predict the fertilization and found at 50% level without a gray zone more useful though it was still insufficient. Conclusion: When we combined the HOS test with CASA parameters, positive predictive value has increased up to 99%. Sensitivity, specificity and negative predictive values also had values over 90%.