3D Printing of Gelatine/Alginate/beta-Tricalcium Phosphate Composite Constructs for Bone Tissue Engineering

Kalkandelen C., Ulag S., Ozbek B., Eroglu G. O., Ozerkan D., Kuruca S. E., ...Daha Fazla

CHEMISTRYSELECT, cilt.4, ss.12032-12036, 2019 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 4
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1002/slct.201902878
  • Dergi Adı: CHEMISTRYSELECT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.12032-12036
  • Anahtar Kelimeler: Alginate, Gelatine, beta-tricalcium phosphate, 3D printing, FABRICATION, SCAFFOLDS
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet

Özet

Bone tissue engineering studies have brought three-dimensional scaffolds into focus that can provide tissue regeneration with designed porosity and strengthened structure. Current research has concentrated on the fabrication of natural and synthetic polymer-based complex structures that closely mimic biological tissues due to their superior biocompatibility and biodegradabilities. Gelatine/Sodium Alginate hydrogels reinforced with different concentrations of beta-Tricalcium Phosphate (TCP) (10, 13, and 15 wt.%) were studied to form 3D bone tissue. Physical, mechanical, chemical, morphological properties and biodegradability of the constructs were investigated. Furthermore, in vitro biological assay with human osteosarcoma cell line (SAOS-2) was performed to determine the biocompatibility of the constructs. It is found that cell viability rates for all constructs were increased and maximum cell viability rate was attained for 20%Gelatine/2%Alginate/10%TCP (wt.). The present work demonstrates that 3D printed Gelatine/Alginate/TCP constructs with porous structures are potential candidates for bone tissue engineering applications.

Bone tissue engineering studies have brought three-dimensional scaffolds into focus that can provide tissue regeneration with designed porosity and strengthened structure. Current research has concentrated on the fabrication of natural and synthetic polymer-based complex structures that closely mimic biological tissues due to their superior biocompatibility and biodegradabilities. Gelatine/Sodium Alginate hydrogels reinforced with different concentrations of β-Tricalcium Phosphate (TCP) (10, 13, and 15 wt.%) were studied to form 3D bone tissue. Physical, mechanical, chemical, morphological properties and biodegradability of the constructs were investigated. Furthermore, in vitro biological assay with human osteosarcoma cell line (SAOS-2) was performed to determine the biocompatibility of the constructs. It is found that cell viability rates for all constructs were increased and maximum cell viability rate was attained for 20%Gelatine/2%Alginate/10%TCP (wt.). The present work demonstrates that 3D printed Gelatine/Alginate/TCP constructs with porous structures are potential candidates for bone tissue engineering applications.