The Effects of Darbepoetin Alfa on the Changes Induced by Ethanol in Glucose Metabolism and Lipid Profile

Uygur E., Seymen H. O., Uzun ., Tunçdemir M., Büge Öz A., Polat E., ...Daha Fazla

Turkish Society of Physiological Sciences, 47th Turkish Physiology Congress, 1-4 November 2022, Antalya, Türkiye, Antalya, Türkiye, 1 - 04 Kasım 2022, ss.47, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Doi Numarası: 10.1111/apha.13905
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.47
  • İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet

Özet

AIM: Ethanol leads to physiopathological disorders by affecting metabolic processes related to glucose metabolism and lipid profile. Darbepoetin alfa (DA) is an analogue of recombinant human erythropoietin (rHuEPO). In our study, we aimed to investigate the effects of darbepoetin alfa (DA) on the changes glucose and lipid metabolism and asprosin levels induced by ethanol in rats. METHODS: 40 Wistar-Albino male rats were divided into four groups of control (C) (3.15 ml/kg isocaloric glucose solution, ig), ethanol (E) (3 g/kg 20% ethanol solution, ig), DA (0.25 µg/kg, ip) and E+DA. Fasting blood glucose (FBG) was measured on the first day, 15th and 30th days. On the 30th day, triglyceride, total cholesterol, HDL and insulin levels in serum and asprosin levels in plasma were measured. Liver tissues were investigated by histopathological methods. RESULTS: FBG was significantly lower in E+DA group on the 30th day compared with C group (p<0.05). Serum insülin and triglyceride levels were significantly higher in E+DA group compared with C and E groups (p<0.05). There was no statistically difference between the groups in HOMA-IR, in the plasma asprosin levels, in the serum total cholesterol and HDL levels. Histopathologically, diffuse microvesicular fat accumulation in hepatocytes, and extensive dilatation and hyperemia in sinusoids were observed in E group compared with C group. In the E+DA group, these pathological findings were significantly reduced. CONCLUSION: In our study, it was observed that ethanol had no effect on asprosin levels, insulin resistance and lipid profile depending on the ethanol dose and ethanol exposure time. In the E+DA group, the decrease in FBG within physiological limits is due to an increase in insulin level. In our study, there was an increase in serum triglyceride levels with the combined effect of ethanol and DA. Keywords: Ethanol, Darbepoetin alpha, Asprosin, Glucose metabolism, Lipid profile.