Dual-mode colorimetric sensing of bilirubin using a PDDA@peroxomolybdate composite: A spectrophotometric platform and cost-effective cotton-swab sensor for point-of-care testing
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, cilt.367, 2027 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 367
- Basım Tarihi: 2027
- Doi Numarası: 10.1016/j.saa.2026.129041
- Dergi Adı: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, Chimica, Compendex, EMBASE, INSPEC, MEDLINE, Academic Search Ultimate (EBSCO), Engineering Source (EBSCO)
- Anahtar Kelimeler: Bilirubin, Colorimetry, Cotton-swab sensor, Naked eye, Peroxomolybdate, Spectrophotometry
- İstanbul Üniversitesi-Cerrahpaşa Adresli: Evet
Özet
In this work, the synthesis and characterization of a poly(diallyldimethylammonium chloride) (PDDA)@peroxomolybdate composite are presented for the development of a dual-mode colorimetric sensing platform for point-of-care bilirubin (BLR) detection. The platform combines a laboratory-based spectrophotometric assay with a smartphone-assisted cotton-swab sensor, enabling versatile and accessible screening. The spectrophotometric mode exhibited a wide linear detection range of 0.1–4.5 mg/dL (1.72–77.0 μM) and a limit of detection (LOD) of 0.032 mg/dL (0.54 μM). In parallel, the smartphone-based assay, utilizing normalized red-channel intensity values, demonstrated a broader linear range of 0.1–9.0 mg/dL (1.72–154 μM) with an LOD of 0.046 mg/dL (0.78 μM). This dual-mode sensing approach offers flexibility by letting users choose between the high analytical accuracy of laboratory instrumentation and the convenience of wide-range, on-site screening, depending on clinical requirements. Furthermore, the selectivity of the platform was comprehensively evaluated, and its practical applicability was successfully validated using adult and neonatal blood serum samples. The results highlight the strong potential of the proposed platform for reliable clinical monitoring of BLR levels.